• Catalog Number:

  E011143-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 312 (A-T-SP-P-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35568

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  IRS-1(Phospho-Ser312) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 312.

• References:

  Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76 Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645 Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97(5): 2355-2360 Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645

Western blot analysis of extract from 293 cells, using IRS-1 (Phospho-Ser312) antibody (E011143, Lane 1 and 2).