• Catalog Number:

  E011140-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CHK1 around the phosphorylation site of serine 280 (V-T-SP-G-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  O14757

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  CHK1 (Phospho-Ser280) antibody detects endogenous levels of CHK1 only when phosphorylated at serine 280.

• References:

  Dephoure N, et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7. Jean C, et al. (2007) J Invest Dermatol. 127(10):2418-24

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using CHK1 (Phospho-Ser280) Antibody (E011140).