• Catalog Number:

  E011136-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FKHR around the phosphorylation site of serine 319 (T-S-SP-N-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q12778

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Alter Name:

  FKHR, FOXO1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma

• Specificity Sensitivity:

  FKHR (phospho-Ser319) antibody detects endogenous levels of FKHR only when phosphorylated at serine 319.

• References:

  Rena G, et al. (2002) EMBO J 21(9): 2263-2271. Woods YL, et al. (2001) Biochem J355(Pt 3): 597-607. Rena G, et al. (2001) Biochem J 354(Pt 3): 605-612.

• All Sites:

  Human: Ser319; Mouse: Ser316; Rat: Ser313

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR (phospho- Ser319) antibody (E011136).

Western blot analysis of extracts from 293 cells (10% serum-treated, 15min) and HeLa cells (EGF-treated, 200ng/ml, 15min), using FKHR (Ab-319) antibody (E021161, Lane 1, 2 and 3) and FKHR (phospho-Ser319) antibody (E011136, Lane 4 and 5).