• Catalog Number:

  E011131-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 807 (Y-I-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06400

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  Rb (phospho-Ser807) antibody detects endogenous levels of Rb only when phosphorylated at serine 807.

• References:

  Roesch A, et al. (2005) Mod Pathol. 18(4): 565-572. Chadee DN, et al. (2004) Nat Cell Biol. 6(8): 770-776. Knudsen ES, et al. (1997) Mol Cell Biol. 17(10): 5771-5783. Knudsen ES, et al. (1996) J Biol Chem. 271(14): 8313-8320.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Rb (phospho-Ser807) antibody (E011131).

Western blot analysis of extracts from K562 cells untreated or treated with 10% serum after 48 hours of starvation, using Rb (phospho-Ser807) antibody (E011131).