• Catalog Number:

  E011130-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 795 (P-S-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06400

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  Rb (phospho-Ser795) antibody detects endogenous levels of Rb only when phosphorylated at serine 795.

• References:

  Rubin SM, et al. (2005) Cell.123 (6): 1093-1106. Wallick CJ, et al. (2005) Oncogene.24 (36): 5606-5618. Leibundgut K, et al. (2005) Stem Cells. 23(7):1002-1011. Roesch A, et al. (2005) Mod Pathol.18 (4): 565-572.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Rb (phospho- Ser795) antibody (E011130).

Western blot analysis of extracts from K562 cells untreated or treated with 10% serum after 48 hours of starvation, using Rb (phospho-Ser795) antibody (E011130).