• Catalog Number:

  E011129-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IKKα around the phosphorylation site of threonine 23 (L-G-TP-G-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O15111

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  IKKα (phospho-Thr23) antibody detects endogenous levels of IKKα only when phosphorylated at threonine 23.

• References:

  Yuan ZQ, et al.(2002) J Biol Chem; 277(33): 29973-82. Ozes ON, et al. (1999) Nature; 401(6748): 82-5.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human colon carcinoma tissue, using IKKα (phospho-Thr23) antibody (E011129).

Western blot analysis of 293 cell extracts treated with EGF using IKKα (phospho-Thr23) antibody (E011129).