Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human HDAC8 around the phosphorylation site of serine 39 (R-A-SP-M-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q9BY41
Applications:
IHC: 1:50~1:100
Specificity Sensitivity:
HDAC8 (phospho-Ser39) antibody detects endogenous levels of HDAC8 only when phosphorylated at serine 39.
References:
Lee H, et al. (2004) Mol Cell Biol. 24(2): 765-773.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using HDAC8 (phospho-Ser39) antibody (E011128).