• Catalog Number:

  E011126-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human LIMK1 around the phosphorylation site of threonine 508 (R-Y-TP-V-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P53667

• Applications:

  WB: 1:500~1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  LIMK1 (phospho-Thr508) antibody detects endogenous levels of LIMK1 only when phosphorylated at threonine 508.

• References:

  Soosairajah J, et al. (2005) EMBO J. Ohashi K, et al. (2000) J Biol Chem; 275(5): 3577-82. Edwards DC, et al. (1999) Nat Cell Biol; 1(5): 253-9.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using LIMK1 (phospho- Thr508) antibody (E011126).

Western blot analysis of extract from NIH/3T3 cells treated or untreated with UV (60min), using LIMK1 (phospho- Thr508) antibody (E011126).