• Catalog Number:

  E011124-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-SP-Y-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P31751

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  Akt2 (phospho-Ser474) antibody detects endogenous levels of Akt2 only when phosphorylated at serine 474.

• References:

  Sun M, et al. (2001) Cancer Res; 61(16): 5985-91. Yuan ZQ, et al. (2000) Oncogene; 19(19): 2324-30. Meier R, et al. (1997) J Biol Chem; 272(48): 30491-7.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2 (phospho- Ser474) antibody (E011124).

| Western blot analysis using Akt2 (Ab-474) antibody (E021155, Lane 1, 2, 3 and 4) and Akt2 (phospho- Ser474) antibody (E011124, Lane 5, 6 and 7).