• Catalog Number:

  E011121-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 345 (S-F-SP-Q-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O14757

• Applications:

  IHC: 1:50~1:100

• Alter Name:

  CHEK1, Chk1, kinase Chk1

• Specificity Sensitivity:

  Chk1 (phospho-Ser345) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345.

• References:

  Shiromizu T, et al. (2006) Genes CellsMay: 11(5): 477-85. Hang YW, et al. (2005) Mol Cell: 19(5): 607-18. Lu X, et al. (2005) Genes Dev: 19(10): 1162-74. Falck J, et al. (2005) Nature: 434(7033): 605-11.

• All Sites:

  Human: Ser345; Mouse: Ser345; Rat: Ser345

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Chk1 (phospho-Ser345) antibody (E011121).