Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Androgen Receptor around the phosphorylation site of serine 213 (E-A-SP-G-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
P10275
Applications:
WB:1:500~1000
Specificity Sensitivity:
Androgen Receptor (phospho-Ser213) antibody detects endogenous levels of Androgen Receptor only when phosphorylated at serine 213.
References:
Brinkman, A.O. et al. (1999) J. Steroid. Biochem. Mol. Biol. 69, 307-313.
Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol. 76, 135-142.
Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146.
Western blot analysis of extract from DU145 cell, using Androgen Receptor (phospho-Ser213) antibody (E011119).