• Catalog Number:

  E011118-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human cdc25C around the phosphorylation site of serine 216 (S-P-SP-M-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P30307

• Applications:

  IHC: 1:50~1:100 IF: 1:100-200

• Alter Name:

  CDC25M1, Dual specificity phosphatase Cdc25C, M-phase inducer phosphatase 3, MPIP3

• Specificity Sensitivity:

  cdc25C (phospho-Ser216) antibody detects endogenous levels of cdc25C only when phosphorylated at serine 216.

• References:

  Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3(4): 341-348. Ferguson AM. et al. (2005) Mol Cell Biol. 25(7): 2853-2860. Donzelli M. et al. (2003) EMBO Rep. 4(7): 671-677. Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99(4): 1990-1995.

• All Sites:

  Human: Ser216

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C (phospho-Ser216) antibody (E011118).