• Catalog Number:

  E011117-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1524 (Y-P-SP-Q-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P38398

• Applications:

  IHC: 1:50~1:100

• Specificity Sensitivity:

  BRCA1 (phospho-Ser1524) antibody detects endogenous levels of BRCA1 only when phosphorylated at serine 1524.

• References:

  Lin SY, et al. (2004) Proc Natl Acad Sci U S A, 101(17): 6484-6489 Zhang J, et al. (2004) Mol Cell Biol; 24(2): 708-718 Beger C, et al. (2001) Proc Natl Acad Sci U S A; 98(1): 130-135 Gardner K, et al. (2001) Breast Cancer Res; 3(1): 11-13 Zheng L, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9587-9592

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using BRCA1 (phospho-Ser1524) antibody (E011117).