• Catalog Number:

  E011115-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FKHR around the phosphorylation site of serine 256 (A-A-SP-M-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q12778

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  FKHR (phospho-Ser256) antibody detects endogenous levels of FKHR only when phosphorylated at serine 256.

• References:

  Gan L, et al. (2005) J Neurochem; 93(5): 1209-19. Smith WW, et al. (2005) J Cell Biol; 169(2): 331-9. Di Maira G, et al. (2005) Cell Death Differ; 12(6): 668-77. Horn S, et al. (2004) Leukemia; 18(11): 1839-49.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using FKHR (phospho-Ser256) antibody (E011115).

HeLa| NIH/3T3 |HeLa Western blot analysis using FKHR (Ab-256) antibody (E021138, Lane 1, 2, 3 and 4) and FKHR (phospho- Ser256) antibody (E011115, Lane 5, 6, 7 and 8).