• Catalog Number:

  E011114-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Myosin Light Chain 2 around the phosphorylation site of serine 18 (A-T-SP-N-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P78527

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  Myosin Light Chain 2 (Phospho-Ser18) Antibody detects endogenous levels of Myosin Light Chain 2 only when phosphorylated at serine 18.

• References:

  Janiak A, et al. (2006) Mol Biol Cell. Apr; 17(4): 1606-1619. Croft DR, et al. (2006) Mol Cell Biol. 2 Jun; 26(12): 4612-4627 Li Z, et al. (2006) Mol Cell Biol. Jun; 26(11): 4240-4256

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myosin Light Chain 2 (Phospho-Ser18) Antibody (E011114).

Western blot analysis of extracts from mouse heart tissue using Myosin Light Chain 2 (Phospho-Ser18) Antibody (E011114).