Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from mouse p90RSK around the phosphorylation site of serine 352 (R-D-SP-P-G).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P18653
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
p90RSK (Phospho-Ser352) Antibody detects endogenous levels of p90RSK only when phosphorylated at serine 352.
References:
Silverman E, et al. Mol Cell Biol. 2004 Dec; 24(24): 10573-10583.
Itoh S, et al. J Biol Chem 2005 Jun 24; 280(25): 24135-24142
Eisenmann KM, et al. Cancer Res 2003 Dec 01; 63(23): 8330-7
Western blot analysis of extract from HUVEC cells using p90RSK (Phospho-Ser352) Antibody (E011113, Lane 1 and 2).