• Catalog Number:

  E011112-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of serine 404 (D-T-SP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P10636

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  Tau (phospho-Ser404) antibody detects endogenous levels of Tau only when phosphorylated at serine404.

• References:

  Li G, Yin H, et.al .(2004) J Biol Chem ; 279(16): 15938-45. Noble W, et al. (2003) Neuron ; 38(4): 555-65. Giasson BI, et al.(2002).. Biochemistry; 41(51): 15376-87. Lee G., et.al. (1989). Neuron 2:1615-1624. Andreadis A.,et.al. (1992) Biochemistry 31:10626-10633.

Western blot analysis of extract from mouse brain tissue using Tau (Ab-404) antibody (E021101, Lane 1 and 2) and Tau (phospho-Ser404) antibody (E011112, Lane 3 and 4).