• Catalog Number:

  E011110-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of threonine 231 (V-R-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P10636

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  Tau (phospho-Thr231) antibody detects endogenous levels of Tau only when phosphorylated at threonine 231.

• References:

  Yen S.H.et.al. (1997) Neurochem. 69:1709-1719. Beausoleil S.A.et.al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135. Wintjens R.et.al. (2001) Biol. Chem. 276:25150-25156. Babu J.R.et.al.(2005) Neurochem. 94:192-203. Goedert M., Spillantini M.G. (2000) Biochim. Biophys. Acta 1502:110-121.

Western blot analysis of extract from mouse brain tissue using Tau (Ab-231) Antibody (E021099, Lane 1 and 2) and Tau (phospho-Thr231) antibody (E011110, Lane 3 and 4).