• Catalog Number:

  E011105-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from mouse p90RSK around the phosphorylation site of threonine 348 (S-R-TP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q15418

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  p90RSK (phospho-Thr348) antibody detects endogenous levels of p90RSK only when phosphorylated at threonine 348.

• References:

  Silverman E, et al. Mol Cell Biol. 2004 Dec; 24(24): 10573-10583. Andrew D, et al. Biochem J. 2006 February 1; 393(Pt 3): 715–724. Itoh S, et al. J Biol Chem 2005 Jun 24; 280(25): 24135-24142

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p90RSK (phospho-Thr348) antibody (E011105).

Western blot analysis of extract from HeLa cells, untreated or treated with PMA (200nM, 30min), using p90RSK (Ab-348) antibody (E021135, Lane 1 and 2) and p90RSK (phospho-Thr348) antibody (E011105, Lane 3 and 4).