Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human NMDAR1 around the phosphorylation site of serine 896 (R-R-SP-S-K).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q05586
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
NMDAR1 (phospho-Ser896) antibody detects endogenous levels of NMDAR1 only when phosphorylated at serine 896.
References:
Tyszkiewicz JP, et al. J Physiol. 2004 Feb 1; 554(Pt 3): 765-777
Western blot analysis of extract from MCF7 cells, untreated or treated with estradiol (100nM, 20min), using NMDAR1 (phospho-Ser896) antibody (E011104).