• Catalog Number:

  E011098-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 37 (L-P-SP-Q-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P04637

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  p53 (phospho-Ser37) antibody detects endogenous levels of p53 only when phosphorylated at serine 37.

• References:

  Ito, A. et al. (2001) EMBO J. 20, 1331-1340. Sakaguchi, K. et al. (1998) Genes Dev. 12, 2831-2841. Solomon, J.M. et al. (2006) Mol. Cell. Biol. 26, 28-38.

Western blot analysis of extract from HT-29 cell untreated or treated with Doxorubicin (1mM, 30min), using p53 (Ab-37) Antibody (E021089, Lane 1 and 2) and p53 (phospho-Ser37) antibody (E011098, Lane 3 and 4).