• Catalog Number:

  E011096-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKD/PKCμ around the phosphorylation site of serine 910 (R-V-SP-I-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q15139

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  PKD/PKCμ (phospho-Ser910) antibody detects endogenous levels of PKD/PKCμ only when phosphorylated at serine 910.

• References:

  Matthews SA, et al. J Biol Chem 1999 Sep; 274(37): 26543-26549 Brandlin I, et al. J Biol Chem 2002 Feb; 277(8): 6490-6496 Storz P, et al. Mol Pharmacol 2004 Oct; 66(4): 870-879

Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 10min), using PKD/PKCμ (Ab-910) antibody (E021128, Lane 1 and 2) and PKD/PKCμ (phospho-Ser910) antibody (E011096, Lane 3 and 4).