• Catalog Number:

  E011095-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of threonine 18 (Q-E-TP-F-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P04637

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  p53 (Phospho-Thr18) antibody detects endogenous levels of p53 only when phosphorylated at threonine 18.

• References:

  Lin T, et al. (2005) Nat Cell Biol; 7(2): 165-71. Vega FM, et al. (2004) Mol Cell Biol; 24(23): 10366-80. Li J, et al. (2004) J Biol Chem; 279(40): 41275-9. Wang J, et al. (2004) J Biol Chem; 279(38): 39584-92.

Western blot analysis of extracts from HT-29 cells untreated or treated with hydroxyurea, using p53 (Ab-18) antibody (E021086, Lane 1 and 2) and p53 (phospho-Thr18) antibody (E011095, Lane 3 and 4).