• Catalog Number:

  E011082-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human G3BP-1 around the phosphorylation site of serine 232 (S-S-SP-P-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q13283

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200

• Specificity Sensitivity:

  G3BP-1 (phospho-Ser232) antibody detects endogenous levels of G3BP-1 only when phosphorylated at serine 232.

• References:

  Tao WA, et al. (2005) Nat Methods. 2(8): 591-598. Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730. Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A.101 (33): 12130-12135. Tourrihre H, et al. (2003) J Cell Biol.160 (6): 823-831.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1 (phospho-Ser232) antibody (E021102).

Western blot analysis of extracts from 293 cells using G3BP-1 (Ab-232) antibody (E021102, Lane 1 and 2) and G3BP-1 (phospho-Ser232) antibody (E011082, Lane 3 and 4).