• Catalog Number:

  E011081-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine1092 (P-E-YP-I-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P00533

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  EGFR (phospho-tyr1092) antibody detects endogenous levels of EGFR only when phosphorylated at tyrosine 1092.

• References:

  Buerger C, et al. (2003) J Biol Chem; 278(39): 37610-21. Wang XQ, (2003) J Biol Chem; 278(49): 48770-8. Saito T, et al. (2004) Endocrinology; 145(9): 4232-43. Pao W, et al. (2004) Proc Natl Acad Sci U S A; 101(36): 13306-11.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using EGFR (phospho-Tyr1092) antibody (E011081).

Western blot analysis of extracts from HUVEC cells using EGFR (Ab-1092) antibody (E021074, Lane 1 and 2) and EGFR (phospho-Tyr1092) antibody (E011081, Lane 3 and 4).