• Catalog Number:

  E011077-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MyoD around the phosphorylation site of serine 200 (A-S-SP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P15172

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  MyoD (phospho-Ser200) antibody detects endogenous levels of MyoD only when phosphorylated at serine 200.

• References:

  Emmanuel G, et al. J. Biol. Chem., Jun 2000; 275: 18767 - 18776 Tintignac LA, et al. Mol Cell Biol. 2004 Feb; 24(4): 1809-1821. Kitzmann M, et a. Mol Cell Biol. 1999 Apr; 19(4): 3167-3176.

Western blot analysis of extract from HeLa cells, untreated or treated with vanadate (1mg/ml, 30min), using MyoD (phospho- Ser200) antibody (E011077).