• Catalog Number:

  E011076-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine1221/1222 (N-L-YP-YP -W).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P04626

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200

• Specificity Sensitivity:

  HER2 (phospho-Tyr1221/Tyr1222) antibody detects endogenous levels of HER2 only when phosphorylated at tyrosine 1221/1222.

• References:

  Marone R, et al. (2004) Nat Cell Biol; 6(6): 515-22. Ren Z, et al. (2002) J Biol Chem; 277(41): 38486-93.

Immunofluorescence staining of methanol-fixed MCF7 cells Using HER2 (phospho- Tyr1221/ Tyr1222) antibody (E011076, Red).

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using HER2 (phospho-Tyr1221/Tyr1222) antibody (E011076).

Western blot analysis of extracts from SK-OV3 cells using HER2 (Ab-1221/1222) antibody (E021071, Line 1 and 2) and HER2 (phospho-Tyr1221/Tyr1222) antibody (E011076, Line 3 and 4).