• Catalog Number:

  E011074-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Progesterone Receptor around the phosphorylation site of serine 190 (G-L-SP-P-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P06401

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200

• Specificity Sensitivity:

  Progesterone Receptor (phospho-Ser190) antibody detects endogenous levels of Progesterone Receptor only when phosphorylated at serine 190.

• References:

  Narayanan R, et al. (2005) Mol Cell Biol; 25(8): 2885-98. Knotts TA, et al. (2001) J Biol Chem; 276(11): 8475-83. Clemm DL, et al. (2000) Mol Endocrinol; 14(1): 52-65. Zhang Y, et al. (1997) Mol Endocrinol; 11(6): 823-32

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using Progesterone Receptor (phospho-Ser190) antibody (E011074).

Western blot analysis of extract from SKOV3 cell untreated or treated with insulin, using Progesterone Receptor (Ab- 190) antibody (E021069, Lane 1 and 2) and Progesterone Receptor (phospho-Ser190) antibody (E011074, Lane 3 and 4).