Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human BCL-XL around the phosphorylation site of serine 62 (A-D-SP-P-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q07817
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200
Specificity Sensitivity:
BCL-XL (phospho-Ser62) antibody detects endogenous levels of BCL-XL only when phosphorylated at serine 62.
References:
Basu A, et.al.( 2003) Mar 13; 538(1-3): 41-7
Cheng E.H.-Y.,et.al. (1996) Nature 379:554-556.
Sattler M.et.al. (1997), Science 275:983-986.
Muchmore S.W.et.al (1996).Nature 381:335-341
Yu J., Zhang L. Mol. (2001)Cell 7:673-682
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-XL (phospho-Ser62) antibody (E011066).
Western blot analysis using BCL-XL (phospho- Ser62) antibody (E011066).