• Catalog Number:

  E011065-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of serine 70 (R-T-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P10415

• Applications:

  IHC: 1:50~1:100 ELISA: 1:10000

• Specificity Sensitivity:

  BCL-2 (phospho-Ser70) antibody detects endogenous levels of BCL-2 only when phos- phorylated at serine 70.

• References:

  Jin Z, Gao F,et al. J Biol Chem 2004 Sep 17; 279(38): 40209-19. Kumar Biswas S, et al. Mol Cancer Ther 2004 Mar; 3(3): 327-34. Huang ST, et al. (2002) FASEB J; 16(8): 825-32. Yamamoto K, et al. (1999) Mol Cell Biol; 19(12): 8469-78.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using BCL-2 (phospho-Ser70) antibody (E011065).