• Catalog Number:

  E011063-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 353 (Q-D-YP-E-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P15311

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Alter Name:

  Cytovillin, EZRI, VIL2, Villin 2, p81

• Specificity Sensitivity:

  Ezrin (phospho-Tyr353) antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 353.

• References:

  Zhao H, et al. (2004) Proc Natl Acad Sci U S A101 (25): 9485-9490. Wang Q, et al. (2003) J Biol Chem 278(48): 47731-47743. Gautreau A, et al. (1999) Proc Natl Acad Sci U S A 96(13): 7300-7305. Crepaldi T, et al. (1997) J Cell Biol 138(2): 423-34.

• All Sites:

  Human: Tyr353; Mouse: Tyr353

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Ezrin (phospho-Tyr353) antibody (E011063).

Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30min), using Ezrin (Ab-353) antibody (E021094, Lane 1 and 2) and Ezrin (phospho-Tyr353) antibody (E011063, Lane 3 and 4).