Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human p73 around the phosphorylation site of tyrosine 99 (S-P-YP-A-Q).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
O15350
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
p73 (phospho-Tyr99) antibody detects endogenous levels of p73 only when phosphorylated at tyrosine 99.
References:
Yuan ZM, et al.(1999) Nature.399(6738): 814-817.
Western blot analysis of extracts from K562 cells using p73 (Ab-99) antibody (E021075, Lane1 and 2) and p73 (phospho-Tyr99) antibody (E011058, Lane 3 and 4).