• Catalog Number:

  E011054-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-SP-Y-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P31749

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  Akt (phospho-Ser473) antibody detects endogenous levels of Akt only when phosphorylated at serine 473.

• References:

  Baudhuin LM, et al. (2004) FASEB J Feb; 18(2): 341-3. Min YH, et al. (2004) Cancer Res; 64(15): 5225-31. Feng J, et al. (2004) J Biol Chem; 279(34): 35510-7.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using Akt (phospho-Ser473) antibody (E011054).

Western blot analysis of extract from HeLa cells untreated or treated with heat shock using Akt (Ab-473) antibody (E021054, Lane 1 and 2) and Akt (phospho-Ser473) antibody (E011054, Lane 3 and 4).