• Catalog Number:

  E011042-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GATA1 around the phosphorylation site of serine 310 (K-A-SP-G-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P15976

• Applications:

  WB: 1:500~1:1000 IF:1:100~1:200

• Specificity Sensitivity:

  GATA1 (phospho-Ser310) antibody detects endogenous levels of GATA1 only when phosphorylated at serine 310.

• References:

  Kadri Z, et al.(2005)Mol Cell Biol 2005 Sep; 25(17): 7412-22.

Western blot analysis of extract from COS7 cells untreated or treated with EPO using GATA1 (Ab-310) antibody (E021042, Line 1 and 2) and GATA1 (phospho-Ser310) antibody (E011042, Line 3 and 4).

Immunofluorescence staining of methanol-fixed HeLa cells using GATA1 (phospho-Ser310) antibody (E011042, Red).