• Catalog Number:

  E011032-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ATF-2 around the phosphorylation site of threonine 73 or 55 (T-P-TP-R-F).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P15336

• Applications:

  IHC: 1:50~1:100 IF:1:100~1:200

• Specificity Sensitivity:

  ATF-2 (phospho-Thr73 or 55) antibody detects endogenous levels of ATF-2 only when phosphorylated at threonine 73 or 55.

• References:

  Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393. Van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811. Livingstone C, et al. (1995) EMBO J. 14: 1785-1797.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF-2 (phospho-Thr73 or 55) antibody (E011032).

Immunofluorescence staining of methanol-fixed HeLa cells using ATF-2 (phospho-Thr73 or 55) antibody (E011032, Red).