• Catalog Number:

  E011027-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human JunB around the phosphorylation site of serine 259 (P-V-SP-P-I).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P17275

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  JunB (phospho-Ser259) antibody detects endogenous levels of JunB only when phosphorylated at serine 259.

• References:

  Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma using JunB (phospho-Ser259) antibody (E011027).

Western blot analysis of extracts from HeLa cells using JunB (phospho-Ser259) antibody (E011027).