Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human c-Jun around the phosphorylation site of serine 243 (P-L-SP-P-I).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against nonphosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P05412
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
Specificity Sensitivity:
c-Jun (phospho-Ser243) antibody detects endogenous levels of c-Jun only when phosphorylated at serine 243.
References:
Boyle W J, et al. (1991) Cell. 64(3): 573-584.
Binetruy B, et al. (1991) Nature. 351: 122-127.
Smeal T, et al. (1991) Nature. 354:494-496.
Derijard B, et al. (1994) Cell. 76:1025-1037.
Kyriakis J M, et al. (1994) Nature. 369: 156-160.