• Catalog Number:

  E011013-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NF-κB around the phosphorylation site of serine 468 (L-A-SP-V-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q04206

• Applications:

  WB: 1:500~1:1000 IF:1:100~1:200

• Specificity Sensitivity:

  NF-κB p65 (phospho-Ser468) antibody detects endogenous levels of NF-κB p65 only when phosphorylated at serine 468.

• References:

  Buss H, et al. (2004) J Biol Chem. Schmitz ML, et al. (2004) Blood.

Western blot analysis of extracts using NF-κB p65 (phospho-Ser468) antibody (E011013).

Immunofluorescence staining of methanol-fixed HeLa cells using NF-κB p65 (phospho-Ser468) antibody (E011013, Red).