• Catalog Number:

  E1A0972A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKA-R2β around the phosphorylation site of serine 113 (R-A-SP-V-C).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P31323

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  cAMP-dependent protein kinase type II-beta regulatory chain; KAP3; PKA R2-beta; PRKAR2B

• Specificity Sensitivity:

  PKA-R2β (Phospho-Ser113) antibody detects endogenous levels of PKA-R2β only when phosphorylated at serine 113.

• References:

  Levy F.O., Mol. Endocrinol. 2:1364-1373(1988). DeGiorgis J.A., J. Proteome Res. 4:306-315(2005). Han G., Proteomics 8:1346-1361(2008).

• All Sites:

  Human: Ser113; Mouse: Ser113; Rat: Ser113

Western blot analysis of extracts from COS-7 cells, treated with PMA (125ng/ml, 30mins), using PKA-R2β (Phospho-Ser113) antibody.