• Catalog Number:

  E1A0970A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PDGFR β around the phosphorylation site of tyrosine 740 (G-G-YP-M-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%),Mouse (Identities = 100%, Positives = 100%); Rat: (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P09619

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  CD140b; EC 2.7.10.1; kinase PDGFR-beta; PDGF-R-beta; PDGFR; PDGFR-beta; PGFRB; platelet-derived growth factor receptor, beta polypeptide

• Specificity Sensitivity:

  PDGFR β (Phospho-Tyr740) antibody detects endogenous levels of PDGFR β only when phosphorylated at tyrosine 740.

• References:

  Gronwald R.G.K., Proc. Natl. Acad. Sci. U.S.A. 85:3435-3439(1988). Claesson-Welsh L., Mol. Cell. Biol. 8:3476-3486(1988). Chi K.D., Oncogene 15:1051-1057(1997).

• All Sites:

  Human: Tyr740; Mouse: Tyr739; Rat: Tyr739

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PDGFR β (Phospho-Tyr740) antibody. Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using PDGFR β (Phospho-Tyr740) antibody.