• Catalog Number:

  E1A0965A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p27 Kip1 around the phosphorylation site of threonine 198 (R-R-R-Q-TP).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P46527

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  CDKN1B, CDN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, KIP1

• Specificity Sensitivity:

  p27 Kip1 (Phospho-Thr198) antibody detects endogenous levels of p27 Kip1 only when phosphorylated at threonine 198.

• References:

  Polyak K., Cell 78:59-66(1994). Pietenpol J.A., Cancer Res. 55:1206-1210(1995). Porter L.A., Mol. Biol. Cell 14:3664-3674(2003).

• All Sites:

  Human: Thr198

Western blot analysis of extracts from HeLa cells, HepG2 cells and COLO205 cells, using p27 Kip1 (Phospho-Thr198) antibody.