Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human JIP1around the phosphorylation site of threonine 103 (G-D-TP-P-G).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q9UQF2
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Other Names:
C-jun-amino-terminal kinase interacting protein 1; IB-1; IB1; Islet-brain-1; JIP-1; JIP-1 related protein; JNK MAP kinase scaffold protein 1; JNK-interacting protein 1; JRP; MAPK8IP; MAPK8IP1; Mitogen-activated protein kinase 8-interacting protein 1; PRKM8IP
Specificity Sensitivity:
JIP1 (Phospho-Thr103) antibody detects endogenous levels of JIP1 only when phosphorylated at threonine 103.
References:
Mooser V., Genomics 55:202-208(1999).
Meyer D., J. Biol. Chem. 274:35113-35118(1999).
Verhey K.J., J. Cell Biol. 152:959-970(2001).
All Sites:
Human: Thr103; Mouse: Thr103; Rat: Thr103
P-peptide - +
Western blot analysis of extracts from COLO205 cells, treated with Serum (20%, 15mins), using JIP1 (Phospho-Thr103) antibody.
Immunofluorescence analysis of NIH/3T3 cells, using JIP1 (Phospho-Thr103) antibody.