• Catalog Number:

  E1A0949A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Histone H4 around the phosphorylation site of serine 47 (R-I-SP-G-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P62805

• Applications:

  IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  H4F2, HIST, HIST1H4A, Histone H4

• Specificity Sensitivity:

  Histone H4 (Phospho-Ser47) antibody detects endogenous levels of Histone H4 only when phosphorylated at serine 47.

• References:

  Elisheva Smith, Nucleic Acids Res., Mar 2005; 33: 1298 - 1308. Lynn Glowczewski, Mol. Cell. Biol., Dec 2004; 24: 10180 - 10192. Partha Mitra, Mol. Cell. Biol., Nov 2003; 23: 8110 - 8123. S Y Roth, Genes & Dev., Mar 1992; 6: 411 - 425.

• All Sites:

  Human: Ser47; Mouse: Ser47

P-peptide - + Immunofluorescence analysis of HuvEc cells, using Histone H4 (Phospho-Ser47) antibody.