• Catalog Number:

  E1A0948A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Histone H1 around the phosphorylation site of threonine 17 (E-K-TP-P-V).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P16401/P16402/P10412

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

   H1, H1 histone family, member 5, H1.5, H15, H1F5, HIST1H1B, histone 1, histone H1.5, histone H1a

• Specificity Sensitivity:

  Histone H1 (Phospho-Thr17) antibody detects endogenous levels of Histone H1 only when phosphorylated at threonine 17.

• References:

  Albig W., Gene 184:141-148(1997). Marzluff W.F., Genomics 80:487-498(2002). Olsen J.V., Cell 127:635-648(2006).

• All Sites:

  Human: Thr17; Mouse: Thr17

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Histone H1 (Phospho-Thr17) antibody. Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using Histone H1 (Phospho-Thr17) antibody.

serum + - Immunofluorescence analysis of HuvEc cells, treated with serum (20%, 30mins), using Histone H1 (Phospho-Thr17) antibody.