• Catalog Number:

  E1A0947A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HER4/ErbB4 around the phosphorylation site of tyrosine 1284 (P-E-YP-L-S).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q15303

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  EC 2.7.10.1, ERBB4, HER4, kinase ErbB4, p180erbB4

• Specificity Sensitivity:

  HER4/ErbB4 (Phospho-Tyr1284) antibody detects endogenous levels of HER4/ErbB4 only when phosphorylated at tyrosine 1284.

• References:

  Plowman G.D., Proc. Natl. Acad. Sci. U.S.A. 90:1746-1750(1993). Elenius K., J. Biol. Chem. 272:26761-26768(1997).

• All Sites:

  Human: Tyr1284; Mouse: Tyr493; Rat: Tyr1284

P-peptide - + Western blot analysis of extracts from HUVEC cells, treated with EGF (200ng/ml, 30mins), using HER4/ErbB4 (Phospho-Tyr1284) antibody. Immunofluorescence analysis of COS7 cells, using HER4/ErbB4 (Phospho-Tyr1284) antibody.