• Catalog Number:

  E1A0944A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HER3/ErbB3 around the phosphorylation site of tyrosine 1222 (L-G-YP-E-Y).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P21860

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  EC 2.7.10.1, ERB3, ERBB3, HER3, Receptor protein-tyrosine kinase erbB-3 precursor, Tyrosine kinase-type cell surface receptor HER3, c-erbB3, kinase ErbB3

• Specificity Sensitivity:

  HER3/ErbB3 (Phospho-Tyr1222) antibody detects endogenous levels of HER3/ErbB3 only when phosphorylated at tyrosine 1222.

• References:

  Katoh M., Biochem. Biophys. Res. Commun. 192:1189-1197(1993). Kinugasa Y., Biochem. Biophys. Res. Commun. 321:1045-1049(2004). Narkis G., Am. J. Hum. Genet. 81:589-595(2007).

• All Sites:

  Human: Tyr1222; Mouse: Tyr1219; Rat: Tyr1219

Western blot analysis of extracts from HUVEC cells, treated with EGF (200ng/ml, 30mins), using HER3/ErbB3 (Phospho-Tyr1222) antibody.

P-peptide - + Immunofluorescence analysis of HuvEc cells, using HER3/ErbB3 (Phospho-Tyr1222) antibody.