• Catalog Number:

  E1A0941A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HDAC6 around the phosphorylation site of serine 22 (P-Q-SP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q9UBN7

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  HD6, HDA6, Histone deacetylase 6, JM21

• Specificity Sensitivity:

  HDAC6 (Phospho-Ser22) antibody detects endogenous levels of HDAC6 only when phosphorylated at serine 22.

• References:

  Grozinger C.M., Proc. Natl. Acad. Sci. U.S.A. 96:4868-4873(1999). Amann J.M., Mol. Cell. Biol. 21:6470-6483(2001). Gao L., J. Biol. Chem. 277:25748-25755(2002). Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).

• All Sites:

  Human: Ser22; Mouse: Ser21

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human testis tissue using HDAC6 (Phospho-Ser22) antibody. Western blot analysis of extracts from NIH/3T3 cells, treated with Anisomycin (25μg/ml, 30mins), using HDAC6 (Phospho-Ser22) antibody.

P-peptide - + Immunofluorescence analysis of HepG2 cells, using HDAC6 (Phospho-Ser22) antibody.