• Catalog Number:

  E1A0939A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HDAC3 around the phosphorylation site of serine 424 (K-E-SP-D-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O15379

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  HD3, Histone deacetylase 3, RPD3-2, SMAP45

• Specificity Sensitivity:

  HDAC3 (Phospho-Ser424) antibody detects endogenous levels of HDAC3 only when phosphorylated at serine 424.

• References:

  Dangond F., Biochem. Biophys. Res. Commun. 242:648-652(1998). Yang W.-M., J. Biol. Chem. 272:28001-28007(1997). Emiliani S., Proc. Natl. Acad. Sci. U.S.A. 95:2795-2800(1998).

• All Sites:

  Human: Ser424; Mouse: Ser424; Rat: Ser424

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human skin tissue using HDAC3 (Phospho-Ser424) antibody. Western blot analysis of extracts from NIH/3T3 cells, using HDAC3 (Phospho-Ser424) antibody.

P-peptide - + Immunofluorescence analysis of A549 cells, using HDAC3 (Phospho-Ser424) antibody.