• Catalog Number:

  E1A0938A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HCK around the phosphorylation site of tyrosine 410 (N-E-YP-T-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P08631

• Applications:

  IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  B-cell/myeloid kinase, BMK, EC 2.7.10.2, Hemopoietic cell kinase, P56-HCK and P60-HCK, Tyrosine-protein kinase HCK, kinase Hck, p59-HCK/p60-HCK

• Specificity Sensitivity:

  HCK (Phospho-Tyr410) antibody detects endogenous levels of HCK only when phosphorylated at tyrosine 410.

• References:

  Ota T., Nat. Genet. 36:40-45(2004). Deloukas P., Nature 414:865-871(2001). Olsen J.V., Cell 127:635-648(2006).

• All Sites:

  Human: Tyr410; Mouse: Tyr408; Rat: Tyr409

P-peptide - + Immunofluorescence analysis of HeLa cells, using HCK (Phospho-Tyr410) antibody.