• Catalog Number:

  E1A0937A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GRK1 around the phosphorylation site of serine 21 (R-G-SP-F-D).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q15835

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  EC 2.7.11.14, RHOK, RK, Rhodopsin kinase, kinase GRK1

• Specificity Sensitivity:

  GRK1 (Phospho-Ser21) antibody detects endogenous levels of GRK1 only when phosphorylated at serine 21.

• References:

  Zhao X., Vis. Neurosci. 14:225-232(1997). Khani S.C., Genomics 35:571-576(1996). Ota T., Nat. Genet. 36:40-45(2004).

• All Sites:

  Human: Ser21; Mouse: Ser21; Rat: Ser21

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using GRK1 (Phospho-Ser21) antibody. Western blot analysis of extracts from COS7 cells, treated with TNF (20ng/ml, 5mins), using GRK1 (Phospho-Ser21) antibody.